Simultaneous determination of gamma-hydroxybutyric acid (GHB) and gamma-butyrolactone (GBL) in beverages

03.02.2010, 16:39
Simultaneous determination of gamma-hydroxybutyric acid (GHB) and gamma-butyrolactone (GBL) in beverages
Drug Facilitated Crime (DFC) and Drug-Facilitated Sexual Assault (DFSA) in particular have become threats to partygoers in nightspots in many places. Prosecution depends on the successful detection of gamma-hydroxybutyrate (GHB) and its prodrug gamma-butyrolactone (GBL), the substances most commonly used to spike beverages to make the victim defenceless. Metrohm offers a straightforward and fast ion-chromatographic method to determine both substances in a single run.
	For the forensic determination of illegally added GHB and GBL in commonly consumed beverages, this method employs direct-injection ion chromatography combined with spectrophotometric detection. In the range of 10 to 200 mg/L, five-point calibration curves for GHB and GBL yield correlation coefficients of 0.9998 and 0.9997, respectively. Spiking experiments in which GHB and GBL were added to alcoholic beverages and non-alcoholic fruit juices showed no matrix effects. The method allows to trace GHB-GBL interconversion, whether in-vivo or in-vitro lactone cleavage or intramolecular GHB esterification, and thus complies with pertinent requirements of law enforcement agencies. Download the technical poster here.

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